Integration host factor and conjugative transfer of the antibiotic resistance plasmid R100.
نویسنده
چکیده
Transfer of plasmid R100-1 was reduced 100-fold in the absence of integration host factor.
منابع مشابه
Swapping single-stranded DNA sequence specificities of relaxases from conjugative plasmids F and R100.
Conjugative plasmid transfer is an important mechanism for diversifying prokaryotic genomes and disseminating antibiotic resistance. Relaxases are conjugative plasmid-encoded proteins essential for plasmid transfer. Relaxases bind and cleave one plasmid strand site- and sequence-specifically before transfer of the cleaved strand. TraI36, a domain of F plasmid TraI that contains relaxase activit...
متن کاملTranslocation of the tetracycline resistance determinant from R100-1 to the Escherichia coli K-12 chromosome.
Pairs of normally incompatible derivatives of R100-1 (one ChlS TetR, the other ChilR TetS) were forced to coexist in a recA host by selection for ChlR TetR cells. After many generations stable derivatives were isolated. The analysis of none independent stabilization experiments showed that in each case TetR was translocated from the plasmid to the chromosome of the host. No evidence for the joi...
متن کاملConjugative transfer in the dissemination of beta-lactam and aminoglycoside resistance.
The dissemination of antibiotic resistance among pathogenic bacteria can be attributed largely to conjugative DNA transfer. The general category of conjugative transfer includes both bacterial plasmid conjugation and the transfer of nonreplicative conjugative transposons. Prototypes for these two systems are the plasmid RK2 and the conjugative transposon Tn916. To address the long-term problem ...
متن کاملThe Bacteroides thetaiotaomicron protein Bacteroides host factor A participates in integration of the integrative conjugative element CTnDOT into the chromosome.
UNLABELLED CTnDOT is a conjugative transposon found in Bacteroides species. It encodes multiple antibiotic resistances and is stimulated to transfer by exposure to tetracycline. CTnDOT integration into the host chromosome requires IntDOT and a previously unknown host factor. We have identified a protein, designated BHFa (Bacteroides host factor A), that participates in integrative recombination...
متن کاملDerepression of conjugal transfer of the antibiotic resistance plasmid R100 by antisense RNA.
Conjugal transfer of the normally repressed antibiotic resistance plasmid R100 was derepressed by fragments of R100 that carried the traJ promoter and the traJ leader but lacked the finP promoter.
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عنوان ژورنال:
- Journal of bacteriology
دوره 169 9 شماره
صفحات -
تاریخ انتشار 1987